Coding
Part:BBa_K3926005:Design
Designed by: Zhipeng Gao Group: iGEM21_XHD-Wuhan-A-China (2021-10-19)
nirK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 40
Illegal NgoMIV site found at 703 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
tga should be cut if you want to link some indicators(eGFP, amilCP etc.)
Source
Sinorhizobium fredii HH103, sequence found through Genbank: https://www.ncbi.nlm.nih.gov/nuccore/NC_016812.1?report=genbank&from=1010580&to=1011710